川滇高山櫟葉片氮磷含量的海拔變化

曹向文; 張淼淼; 陳健; 史作民

doi:10.12356/j.2096-8884.2023-0038

川滇高山櫟葉片氮磷含量的海拔變化

doi: 10.12356/j.2096-8884.2023-0038

曹向文^{1, 2,},
張淼淼^{1, 2},
陳健^{1, 2},
史作民^{1, 2, 3, ,}

1.
中國林業科學研究院森林生態環境與自然保護研究所，國家林業和草原局森林生態環境重點實驗室，北京 100091
2.
四川米亞羅森林生態系統定位觀測研究站，四川理縣 623100
3.
南京林業大學南方現代林業協同創新中心，南京 210037

基金項目: 中央級公益性科研院所基本科研業務費專項資金項目（CAFYBB2021ZA002-2）；國家重點研發計劃（2021YFD2200405）；中央級公益性科研院所基本科研業務費專項資金項目（CAFYBB2022SY021）；中央級公益性科研院所基本科研業務費專項資金項目（CAFYBB2022QC002）

詳細信息

作者簡介:
曹向文：E-mail: caoxiangwen67@163.com

通訊作者:
E-mail: shizm@caf.ac.cn

中圖分類號: S718.5
計量
- 文章訪問數: 37
- HTML全文瀏覽量: 13
- PDF下載量: 4
- 被引次數: 0
出版歷程
- 收稿日期: 2023-05-10
- 錄用日期: 2023-08-22
- 網絡出版日期: 2023-08-29

Variation of N and P Contents of Quercus aquifolioides Leaves Along Elevation

Xiangwen Cao^{1, 2
,},
Miaomiao Zhang^{1, 2},
Jian Chen^{1, 2},
Zuomin Shi^{1, 2, 3
, ,}

1.
Key Laboratory of Forest Ecology and Environment of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing 100091, China
2.
Miyaluo Research Station of Alpine Forest Ecosystem, Lixian County 623100, China
3.
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China

摘要

摘要: 目的研究川滇高山櫟（Quercus aquifolioides）葉片氮和磷含量特征隨海拔的變化規律，了解調控其變化的主要因素，揭示川滇高山櫟葉片對環境變化的響應機制。方法以青藏高原東南部川滇高山櫟林為研究對象，采集不同海拔的川滇高山櫟葉片以及0～10 cm表層土，通過元素分析儀和等離子體發射光譜儀測定葉片氮和磷含量，研究葉片氮和磷含量隨海拔的變化規律，并結合葉片部分性狀特征和土壤理化性質，采用變異分區分析探討影響葉片氮和磷含量變異的主要因子。結果川滇高山櫟葉片單位面積氮含量變化在0.25～0.29 mg·cm^?2之間，單位質量氮含量變化在11.41～14.82 g·kg^?1之間。葉片單位面積磷含量變化在14.83～32.77 μg·cm^?2之間，單位質量磷含量變化在0.74～1.79 g·kg^?1之間。海拔顯著影響川滇高山櫟葉片氮和磷含量，其中葉片單位面積和單位質量的氮含量均呈現沿海拔升高而下降的趨勢，而葉片單位面積和單位質量的磷含量均呈現沿海拔升高而增加的趨勢。同時，海拔也顯著影響部分葉片性狀特征，其中葉鮮重、葉面積和葉密度呈現沿海拔升高而下降的趨勢，葉厚呈現沿海拔升高而增加的趨勢，葉片含水率和單位面積葉質量未發現明顯變化趨勢。此外，葉片氮和磷含量隨葉片性狀變化表現出不同變化趨勢，單位質量氮含量隨單位面積葉質量、葉厚的增加而下降，隨葉密度增加而增加，而單位質量磷含量隨單位面積葉質量、葉密度增加而下降。調控葉片氮和磷含量變化的主控因素存在差異，氮含量主要受葉片性狀、土壤性質及兩者的協同作用共同影響，而磷含量受土壤性質、土壤性質和葉片性狀協同作用的影響更大，受葉片性狀的獨立影響相對較小。結論川滇高山櫟葉片氮含量隨海拔升高而下降，葉片磷含量隨海拔升高而增加，且均受到土壤性質和葉片性狀的影響。因此，在預測葉片氮和磷含量對海拔變化的響應時，要充分考慮葉片性狀、土壤性質的可能影響。
- 海拔 /
- 葉片養分 /
- 空間異質性 /
- 土壤性質 /
- 青藏高原 /
- 川滇高山櫟
Abstract: Objective The purpose of the study was to analyze the change patterns of Quercus aquifolioides leaf nitrogen and phosphorus characteristics along elevations, to understand the main factors regulating leaf nitrogen and phosphorus changes, and to reveal the response mechanism of Q. aquifolioides leaves to environmental changes. Method In this study, Q. aquifolioides forests on the southeastern Qinghai-Tibetan Plateau were used as the research object, and the leaves of Q. aquifolioides and 0～10 cm topsoil at different elevations were collected. The nitrogen and phosphorus contents of the leaves were determined by elemental analyzer and plasma emission spectroscopy. The variation patterns of leaf nitrogen and phosphorus contents along different elevations were studied, and the main factors affecting leaf nitrogen and phosphorus contents were explored using variation partitioning analysis in combination with partial leaf trait characteristics and soil physicochemical properties. Result The nitrogen content per unit area of Q. aquifolioides leaves ranged from 0.25～0.29 mg·cm^?2 and nitrogen content per unit mass ranged from 11.41～14.82 g·kg^?1. The phosphorus content per unit area of the leaves ranged from 14.83～32.77 μg·cm^?2 and phosphorus content per unit mass ranged from 0.74～1.79 g·kg^?1. Elevation affected the nitrogen and phosphorus contents of Q. aquifolioides leaves significantly, and the nitrogen content per unit area and per unit mass of leaves decreased with the increase of elevation, while the phosphorus content per unit area and per unit mass showed an increasing trend along with the elevation. Meanwhile, elevation also affected partial leaf trait characteristics significantly. Leaf fresh mass, leaf area and leaf density showed a decreasing trend along the elevation, and leaf thickness showed an increasing trend along the elevation. Leaf moisture and leaf mass per unit area did not show a significant trend along the elevation. In addition, leaf nitrogen and phosphorus contents showed different trends with changes in leaf traits. The unit mass nitrogen content decreased with the increase of leaf mass per unit area and leaf thickness and increased with the increase of leaf density, while the unit mass phosphorus content decreased with the increase of leaf mass per unit area and leaf density. The main controlling factors regulating the changes of leaf nitrogen and phosphorus contents differed, with nitrogen content mainly affected by the combined effect of leaf traits, soil properties and the synergistic effect of both, while phosphorus content was mainly affected by soil properties, the synergistic effect of soil properties and leaf traits, and was relatively less affected by the independent effect of leaf traits. Conclusion The leaf nitrogen content of Q. aquifolioides decreased and the leaf phosphorus content increased with elevation, and both were affected by soil properties and leaf traits. Therefore, the possible effects of leaf traits and soil properties on leaf nutrient contents should be fully considered when predicting the trends of leaf N and P contents in response to elevation changes.
- elevation /
- leaf nutrient /
- spatial heterogeneity /
- soil property /
- the Qinghai-Tibetan Plateau /
- Quercus aquifolioides

HTML全文

圖 1 不同海拔的川滇高山櫟葉片N和P含量

注: （a）不同海拔的葉片單位面積N（N_area）和P（P_area）含量的差異性。（b）不同海拔的葉片單位質量N（N_mass）和P（P_mass）含量的差異性。不同小寫或大寫字母分別表示不同海拔的葉片N含量或P含量差異顯著（P ＜ 0.05）。**，P ＜ 0.01。(a) Variability of N (N_area) and P (P_area) contents per unit area of leaves at different elevations. (b) Variability of N (N_mass) and P (P_mass) contents per unit mass of leaves at different elevations. Different lowercase letters or capital letters indicate significant differences (P ＜ 0.05) in leaf N contents or P contents at different elevations. **，P ＜ 0.01.

Figure 1. Leaf N and P contents of Quercus aquifolioides at different elevations

下載: 全尺寸圖片幻燈片

圖 2 葉片單位質量N、P含量與LMA、LD、LT的關系

Figure 2. Relationship between leaf N and P content per unit mass and LMA, LD, LT

下載: 全尺寸圖片幻燈片

圖 3 不同海拔的川滇高山櫟林內土壤理化性質

Figure 3. Soil physicochemical properties in Quercus aquifolioides forests along elevations

下載: 全尺寸圖片幻燈片

圖 4 不同影響因子對葉片N_mass和P_mass含量的相對貢獻

注: 通過方差分解分析（VPA）確定葉片性狀和土壤性質對葉片N_mass（a）和P_mass（b）含量的相對貢獻。葉片性狀：葉鮮重、葉片含水率、LMA、LT、LA、LD。土壤性質：土壤含水率、pH、TC、TN、TP、TC:TN、TC:TP、TN:TP、NH₄⁺-N、NO₃^?-N、有效P。The relative contributions of leaf traits and soil properties to leaf N_mass (a) and P_mass (b) contents by VPA. Leaf traits: leaf fresh mass, leaf moisture, LMA, LT, LA, and LD. Soil properties: soil moisture, pH, TC, TN, TP, TC:TN, TC:TP, TN:TP, NH₄⁺-N, NO₃^?-N, and available P.

Figure 4. Relative contributions of different impact factors to leaf N_mass and P_mass contents

下載: 全尺寸圖片幻燈片

表 1 不同海拔的川滇高山櫟葉片部分性狀的混合線性模型分析

Table 1. Mixed linear model analysis of partial leaf trait characteristics of Quercus aquifolioides under different elevations

海拔 Elevation/m	葉鮮重 Leaf fresh mass/g	葉片含水率 Leaf moisture/%	葉厚 LT/μm	葉面積 LA/cm²	單位面積葉質量 LMA/(g m^?2)	葉密度 LD/(g cm^?3)
3100	0.42±0.02a	41.43±0.62b	350.67±10.31b	11.82±0.48b	209.95±5.32ab	0.60±0.03a
3250	0.36±0.02b	41.32±0.55b	346.13±3.33b	10.79±0.34b	197.67±2.20abc	0.57±0.01ab
3430	0.46±0.01a	42.18±0.71b	370.67±15.42b	13.42±0.32a	196.79±5.11bc	0.53±0.02abc
3730	0.24±0.01c	45.38±0.56a	360.27±6.71b	7.21±0.26c	183.03±4.67c	0.51±0.01bc
3880	0.25±0.02c	43.26±0.57ab	422.93±13.74a	7.01±0.49cd	199.46±2.62abc	0.47±0.02c
4030	0.19±0.01cd	41.98±0.78b	428.40±7.61a	5.56±0.23de	200.18±3.98abc	0.47±0.01c
4180	0.16±0.01d	42.25±0.69b	423.73±8.24a	4.29±0.21e	214.98±2.99a	0.51±0.01bc
固定效應	77.057^**	4.730^**	13.580^**	97.669^**	6.482^**	8.231^**
注：不同小寫字母表示不同海拔下葉片性狀指標差異顯著（P ＜ 0.05）。，P ＜ 0.01。Different lowercase letters indicate significant differences (P ＜ 0.05) in leaf trait indexes at different elevations. , P ＜ 0.01.

下載: 導出CSV

表 2 川滇高山櫟部分葉片性狀與海拔的線性回歸分析

Table 2. Linear regression analysis between partial leaf traits of Quercus aquifolioides and elevation

變量 Variables	R²	F	P	回歸方程 Regression equations
葉鮮重 Leaf fresh mass	0.787	122.201	＜0.01^**	y = 1.253?0.0003x
葉片含水率 Leaf moisture	—	—	—	—
葉厚 LT	0.594	48.283	＜0.01^**	y = 87.358 + 0.082x
葉面積 LA	0.789	123.098	＜0.01^**	y = 36.588?0.008x
單位面積葉質量 LMA	—	—	—	—
葉密度 LD	0.498	32.696	＜0.01^**	y = 0.914?0.0001x
注：**，P ＜ 0.01。

下載: 導出CSV

表 3 葉片N、P含量在不同海拔下的線性回歸分析

Table 3. Linear regression analysis of leaf N and P contents under different elevations

變量 Variables	R²	F	P	回歸方程 Regression equations
N_area	0.244	10.650	0.003^**	y = 0.375?3.15×10^?5x
N_mass	0.226	9.625	0.004^**	y = 19.108?0.002x
P_area	0.516	35.171	＜0.001^***	y = ?24.506 + 0.013x
P_mass	0.412	23.087	＜0.001^***	y = ?1.151 + 0.001x
注：，P ＜ 0.01；*，P ＜ 0.001。

下載: 導出CSV

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